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JAYPEE JOURNALS
International Scientific Journals from Jaypee
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List of All Articles
1.  Original Research
Thyroid Status in Patients with Dysfunctional Uterine Bleeding in a Tertiary Care Hospital of Assam
Jadab K Phukan, Gautom K Saharia, Rohini Goswami
[Year:2016] [Month:January-June] [Volume:20 ] [Number:1] [Pages:42] [Pages No:11-15] [No of Hits : 559]
Full Text PDF | Abstract | DOI : 10.5005/jp-journals-10054-0003 | FREE

ABSTRACT

Background: Dysfunctional uterine bleeding (DUB) is a major form of abnormal uterine bleeding, seen in at least 10% of all new outpatient department patients. The thyroid gland is known to play an important role in maintaining a healthy menstrual cycle.

Aims: To assess the thyroid hormone status in apparently euthyroid patients with DUB and to correlate it with incidence of DUB.

Materials and methods: Fifty DUB patients were selected on the basis of clinical history, examination, and relevant investigations. Equal numbers of age-matched women with normal menstrual cycle were taken as controls. Thyroid hormones, viz., thyroidstimulating hormone, total triiodothyronine and tetraiodothyronine, were estimated by radioimmunoassay. Statistical analysis of the data was performed by using Microsoft Excel software.

Results: The most common menstrual abnormality was menorrhagia (48%) followed by metrorrhagia and polymenorrhea (14% each). Hypothyroidism was more prevalent among cases (85.7%) as compared with controls (14.3%). In patients with menorrhagia, 33.3% of patients had hypothyroidism.

Conclusion: Hypothyroidism occurs in DUB patients commonly. There is a need for mandatory thyroid screening in all patients with menstrual irregularities to help in early detection of the cause and treatment of DUB patients to avoid surgery.

Keywords: Assam, Dysfunctional uterine bleeding, Hypothyroidism, Menorrhagia.

How to cite this article: Phukan JK, Saharia GK, Goswami R. Thyroid Status in Patients with Dysfunctional Uterine Bleeding in a Tertiary Care Hospital of Assam. Indian J Med Biochem 2016;20(1):11-15.

Source of support: MD/MS Thesis Grant of Rs 1,50,000/- (Rupees one lakh fifty thousand only) for students of North East region from the Department of Biotechnology under Ministry of Science and Technology, Government of India.

Conflict of interest: None

 
2.  Original Research
Alterations in Urinary Microalbumin and Serum Antioxidants in Sickle Cell Disease
PK Patra, PK Khodiar, D Sahu, GK Sahu
[Year:2016] [Month:January-June] [Volume:20 ] [Number:1] [Pages:42] [Pages No:1-5] [No of Hits : 523]
Full Text PDF | Abstract | DOI : 10.5005/jp-journals-10054-0001 | FREE

ABSTRACT

Changes in the level of various biochemical parameters play a significant role in the physiopathology of sickle cell disease (SCD). The aim of this study is to determine the level of urinary micoalbumin and plasma level of ascorbic acid and uric acid in subjects suffering from sickle cell anemia. A total of 30 subjects consisting of both males and females whose age range varied from 10 to 30 years were included in the study. The urinary albumin/creatinine ratio invariably increased in all studied subjects as compared with the control subjects. The level of ascorbic acid in the plasma significantly declined in SCD subjects when compared with that of control subjects (p < 0.05). Also, the decrease in level of uric acid in plasma of SCD patients was significant as compared with the control subjects. Significant changes in these biochemical parameters thus could be used as reliable markers in nephropathy in sickle cell patients and thus in the management of the disease.

Keywords: Ascorbic acid, Microalbuminuria, Nephropathy, Sickle cell disease.

How to cite this article: Patra PK, Khodiar PK, Sahu D, Sahu GK. Alterations in Urinary Microalbumin and Serum Antioxidants in Sickle Cell Disease. Indian J Med Biochem 2016;20(1):1-5.

Source of support: Nil

Conflict of interest: None

 
3.  Original Article
Thyroid Function Status in Indian Adult Nonpregnant Females in Ranchi, India
Barun K Chakrabarty, Binay Mitra, Bhaskar Shahbabu, Nandita Hazra, Sarvinder Singh
[Year:2017] [Month:January-June] [Volume:21 ] [Number:1] [Pages:71] [Pages No:25-29] [No of Hits : 10]
Full Text PDF | Abstract | DOI : 10.5005/jp-journals-10054-0014 | FREE

ABSTRACT

Aim: Thyroid disorders are one of the most common endocrine diseases in India. Thyroid disorders are more common in women than in men and contribute to significant morbidity. In this postiodization era, there is paucity of pan-Indian data of thyroid disorder status among adult nonpregnant women. This study was done to analyze the thyroid hormone levels in women of Jharkhand region, which is traditionally known to be an iodine-deficient area.

Materials and methods: Three hundred and forty nonpregnant adult females in Ranchi area who were consuming iodized salt formed part of the study group. Clinical evaluation was done by a gynecologist pertaining to thyroid illness. Thyroid function tests encompassing triiodothyronine, thyroxine, and thyroidstimulating hormone were carried out by quantitative enzyme immunoassay method. Thyroid status of the population was defined as per kit reference range.

Results: Subjects with age range 20 to 67 years were divided into three groups as per clinical status of thyroid disorder, viz. total, disease free, and control. A total of 19.6% had biochemical evidence of thyroid disorder and 82.4% were euthyroid as per reference ranges in kit literature. Out of hypothyroid subjects, 3.2% had clinical and 14.4% had subclinical hypothyroidism. In the study group, no subjects were detected to have overt or subclinical hyperthyroidism. Multiple comparison analysis was done with Statistical Package for the Social Sciences version 20.0, a statistical software package.

Discussion: This is the first study in Jharkhand area on nonpregnant adult female population that are getting iodine sufficient foods in an iodine-deficient region. The study showed high prevalence of thyroid disorders in the study group. Hypothyroidism, predominantly subclinical hypothyroidism, is prevalent among women in this region.

Keywords: Iodine sufficient, Nonpregnant adult female, Subclinical hypothyroidism, Thyroid disorders.

How to cite this article: Chakrabarty BK, Mitra B, Shahbabu B, Hazra N, Singh S. Thyroid Function Status in Indian Adult Nonpregnant Females in Ranchi, India. Indian J Med Biochem 2017;21(1):25-29.

Source of support: Nil

Conflict of interest: None

 
4.  Original Article
Status and Effect of Vitamin D on Microvascular Complication in Type II Diabetes Mellitus Patients
P Krushna Kishore, Ipsita Choudhary
[Year:2017] [Month:January-June] [Volume:21 ] [Number:1] [Pages:71] [Pages No:34-37] [No of Hits : 5]
Full Text PDF | Abstract | DOI : 10.5005/jp-journals-10054-0016 | FREE

ABSTRACT

Introduction: Vitamin D has a great impact on human health and disease. Recent studies proved that hypovitaminosis D is a possible risk factor for the pathogenesis of diabetes and its complications. In the present study, we evaluated vitamin D levels and its correlation with the duration of diabetes, sex, glycosylated hemoglobin (HbA1C), and microvascular complications in type II diabetes mellitus (T2DM) patients with and without microvascular complications.

Objectives: To study and correlate vitamin D levels and its relation with duration of diabetes, sex, glycemic control, and microvascular complication in T2DM patients with and without microvascular complications.

Materials and methods: Cross-sectional case-control study of 200 patients (35-70 years) suffering from T2DM was included. The T2DM patients without microvascular complications served as control group. Enrolled subjects underwent complete physical examination to detect the presence of microvascular complications, which includes microfilament tests, detailed fundus examination, urinary microalbumin/creatinine ratio. Fasting blood sugar, HbA1C, and serum vitamin D were also measured by using serum sample.

Results: Prevalence of vitamin D deficiency and insufficiency was found to be significantly higher in T2DM patients with microvascular complications (19.54 ± 2.56 vs 28.57 ± 2.93) as compared with T2DM patients without microvascular complications (p < 0.001). Hypovitaminosis D was found to be significantly more in females (60.75%) compared with males (39.96%) in both the study groups (p < 0.0001). Hypovitaminosis D was correlated with glycemic control and prevalence of microvascular complications.

Conclusion: Vitamin D is correlated with glycemic status and sex difference and has a role in pathogenesis of T2DM and its complications.

Keywords: Glycemic control, Microvascular complications, Type II diabetes mellitus, Vitamin D.

How to cite this article: Kishore PK, Choudhary I. Status and Effect of Vitamin D on Microvascular Complication in Type II Diabetes Mellitus Patients. Indian J Med Biochem 2017; 21(1):34-37.

Source of support: Nil

Conflict of interest: None

 
5.  Original Article
Novel Lipid Indices as a Better Marker of Cardiovascular Disease Risk in Postmenopausal Women
Muruga Sirigere, S Meera
[Year:2017] [Month:January-June] [Volume:21 ] [Number:1] [Pages:71] [Pages No:38-41] [No of Hits : 5]
Full Text PDF | Abstract | DOI : 10.5005/jp-journals-10054-0017 | FREE

ABSTRACT

Introduction: Cardiovascular disease (CVD) is the major leading cause of mortality in men and women around the globe. The incidence of CVD increases with age in both the sexes, whereas it has been noted that there is a marked increase in risk among women after menopause. The hormonal changes associated with the menopause like low level of plasma estrogen and marked increase in follicle stimulating hormone levels exert a significant effect on metabolism of plasma lipids and lipoproteins leading to atherosclerosis, thereby increasing the risk of CVD in postmenopausal women.

Objectives: To study the lipid profile parameters and to calculate and compare the lipid indices with lipid profile parameters in pre- and postmenopausal women.

Materials and methods: The study group included 90 women comprising 45 postmenopausal (cases) and 45 premenopausal (control) women. To estimate lipid profile, enzymatic method was used and for calculation of lipid indices appropriate formula was used.

Results: Altered lipid profile was observed in postmenopausal women, but it was not statistically significant. Atherogenic Index of Plasma, Castelli’s risk index-I, atherogenic coefficient, and nonhigh-density lipoprotein cholesterol were significantly increased in postmenopausal women compared with premenopausal women, but there was no significant increase in Castelli’s risk index-II.

Conclusion: Lipid indices may be considered as a better and cost-effective tool in assessing the risk of CVD in postmenopausal women.

Keywords: Cardiovascular disease, Lipid indices, Lipid profile, Postmenopause.

How to cite this article: Sirigere M, Meera S. Novel Lipid Indices as a Better Marker of Cardiovascular Disease Risk in Postmenopausal Women. Indian J Med Biochem 2017;21(1):38-41.

Source of support: Nil

Conflict of interest: None

 
6.  Original Article
Establishment of Reference Intervals of Serum Hepatic Enzymes in Tertiary Care Hospital: A Pilot Study
P Shruthi Rai, Sukanya Shetty, Priya Patil, Roopa Bhandary
[Year:2017] [Month:January-June] [Volume:21 ] [Number:1] [Pages:71] [Pages No:22-24] [No of Hits : 4]
Full Text PDF | Abstract | DOI : 10.5005/jp-journals-10054-0013 | FREE

ABSTRACT

Introduction: Reference intervals are the most common decision- making tools for the interpretation of various biochemical reports. Age, sex, ethnicity, diet, physical, and socioeconomic conditions affect the physiology of population. Hence, it is necessary to set up the reference values that are applicable to a specific population rather than using the reference values established for other population-based studies or from the literature provided by the reagent kit manufacturers.

Aims and objectives: To establish the reference intervals of serum hepatic enzymes in tertiary care hospital.

Materials and methods: Hospital-based cross-sectional study was done involving 200 subjects. The subjects were randomly selected from the population, which included medical students, health professionals, and those attending different outpatient departments and health camps organized by the hospital for general checkups under health plan scheme. Serum alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP) and gamma-glutamyl transferase (GGT) were estimated by the International Federation of Clinical Chemistry-recommended kinetic method and GGT by enzymatic colorimetric method. To obtain reference interval, 97.5th percentile and 2.5th percentile were used.

Results: The results for serum AST and GGT were found to be statistically significant. Hence separate reference intervals for both male and female were set up. Generalized reference intervals were established for serum ALT and ALP levels.

Conclusion: The present study revealed a significant difference in the reference intervals between males and females.

Keywords: Alanine transaminase, Alkaline phosphatase, Aspartate transaminase, Gamma-glutamyl transferase, Hepatic enzymes, Reference interval.

How to cite this article: Rai PS, Shetty S, Patil P, Bhandary R. Establishment of Reference Intervals of Serum Hepatic Enzymes in Tertiary Care Hospital: A Pilot Study. Indian J Med Biochem 2017;21(1):22-24.

Source of support: The authors would like to acknowledge the Indian Council for Medical Research with Ref: No.3/2/May.2015/ PG-Thesis-HRD (1) dated 6/5/2015 for funding this project.

Conflict of interest: None

 
7.  Original Article
Systemic Cytokine Response predicts the Outcome of Patients from Mechanical Ventilation
Akila Prashant, Prashant Vishwanath, Nalini Kotekar, Suma M Nataraj, Caroline Kuruvilla, CS Nagalakshmi, Parveen Doddamani
[Year:2017] [Month:January-June] [Volume:21 ] [Number:1] [Pages:71] [Pages No:5-10] [No of Hits : 4]
Full Text PDF | Abstract | DOI : 10.5005/jp-journals-10054-0010 | FREE

ABSTRACT

Purpose: Despite the lifesaving potential, mechanical ventilation (MV) imposes a considerable amount of mechanical stress on the lung. Pulmonary and systemic cytokine release due to inflammatory process triggered by MV may give valuable information on patient outcome.

Materials and methods: Thirty patients aged >18 years with acute respiratory distress who required MV were enrolled for the study. Three milliliters of the venous blood was collected immediately after the initiation of MV and at 24 hours. Serum levels of interleukin (IL)-6, IL-8 and, tumor necrosis factor-α (TNF-α) were estimated using quantitative immunometric sandwich enzyme immunoassay technique. Based on their outcome from MV, they were divided into two groups: Survivors (group I) and nonsurvivors (group II).

Results: Serum levels of IL-6 and IL-8 significantly increased in group II (n=13) when compared with group I (n = 17) (p < 0.0001 and p < 0.001 respectively) at 24 hours of MV. However, TNF-α did not show any significant difference between the two groups. The IL-6 >111.9 pg/mL at 24 hours of MV increases the probability of mortality by factor 2.40. An increase of IL-6 by 1 pg/mL significantly increases the relative probability of mortality by a factor of 1.004 (95% CI, 1.0003-1.0078, p = 0.0001).

Conclusion: Estimating the levels of IL-6 and IL-8 at 24 hours of connecting the patient to MV will help in predicting the outcome of the patient.

Keywords: Cytokines, Interleukin-6, Interleukin-8, Mechanical ventilation, Tumor necrosis factor-α.

How to cite this article: Prashant A, Vishwanath P, Kotekar N, Nataraj SM, Kuruvilla C, Nagalakshmi CS, Doddamani P. Systemic Cytokine Response predicts the Outcome of Patients from Mechanical Ventilation. Indian J Med Biochem 2017;21(1):5-10.

Source of support: Nil

Conflict of interest: None

 
8.  EDITORIAL
EDITORIAL
Anju jain
[Year:2017] [Month:January-June] [Volume:21 ] [Number:1] [Pages:71] [Pages No:iv-v] [No of Hits : 3]
Full Text PDF | Abstract | FREE

ABSTRACT

The word autophagy originates from the Greek words auto-, meaning “self”, and phagein, meaning “to eat”. Thus, autophagy denotes “self-eating”. This concept emerged during the 1960’s, when researchers first observed that the cell could destroy its own contents by enclosing it in membranes, forming sack-like vesicles that were transported to a recycling compartment, called the lysosome, for degradation.

 
9.  Original Article
Comparison of BiliCare Transcutaneous Bilirubinometer with Standard Laboratory Assay
Jayesh Warade
[Year:2017] [Month:January-June] [Volume:21 ] [Number:1] [Pages:71] [Pages No:1-4] [No of Hits : 3]
Full Text PDF | Abstract | DOI : 10.5005/jp-journals-10054-0009 | FREE

ABSTRACT

Introduction: Jaundice is the visible manifestation of chemical bilirubinemia and is characterized by yellow discoloration of skin. In a pediatric emergency department, evaluation of jaundice includes a precise medical history, physical examination, and estimation of bilirubin by laboratory tests. Measurement of total serum bilirubin (TSB) by transcutaneous bilirubin (TcB) is interpreted according to the age (in hours) of the newborn infant. Studies have also indicated that the TcB determination is more accurate than visual estimation methods.

Aim: To compare BiliCare System against standard laboratory testing for bilirubin estimation.

Results: The coefficient of correlation was r = 0.7245, and the standard deviation between the mean values of TcB and TSB is ±0.55.

Conclusion: It is concluded from the study that there is good correlation between TcB measurement and laboratory estimation of total bilirubin levels. It is recommended that transcutaneous measurement can be used for initial screening of jaundice as well as monitoring of phototherapy in neonates.

Keywords: BiliCare, Bilirubin, Jaundice, Kernicterus, Transcutaneous.

How to cite this article: Warade J. Comparison of BiliCare Transcutaneous Bilirubinometer with Standard Laboratory Assay. Indian J Med Biochem 2017;21(1):1-4.

Source of support: Nil

Conflict of interest: None

 
10.  Original Article
Specific Reference Intervals of Serum Triiodothyronine, Thyroxine, and Thyroid-stimulating Hormone in Normal Pregnant Indian Women as per Trimester
Barun K Chakrabarty, Binay Mitra, Reetika Pal, Nandita Hazra
[Year:2017] [Month:January-June] [Volume:21 ] [Number:1] [Pages:71] [Pages No:17-21] [No of Hits : 3]
Full Text PDF | Abstract | DOI : 10.5005/jp-journals-10054-0012 | FREE

ABSTRACT

Aim: Maternal thyroid hormone levels during pregnancy are vital for the health of the mother as well as the developing child. Fetal growth is affected by maternal thyroid levels. Various physiological changes like alterations of thyroxine-binding globulins, human chorionic gonadotropin level, and changes in iodide metabolism affect maternal thyroid hormone levels. Therefore, reference intervals (RIs) for thyroid hormones in pregnant population require to be established separately from the general population.

Materials and methods: The RIs of serum triiodothyronine (T3), thyroxine (T4), and thyroid-stimulating hormone (TSH) were determined in healthy pregnant women by enzyme-linked immunosorbent assay (ELISA) technique after segregating them into three trimesters. This study was conducted in a 492-bedded zonal-level hospital. The reference population was chosen from a study population of pregnant women by strict inclusion and exclusion criteria. The assays were done by the most-commonly used, economical ELISA method employing standard kits. Tests were done using accurate and precise methods with proper quality control measures.

Results: The RIs were calculated from the central 95% of distribution of total T3, total T4, and TSH values located between 2.5 and 97.5 percentile values. The 0.90 confidence intervals for the upper and lower reference limits were calculated. The values thus obtained were different from those provided by the manufacturer kit literature.

Conclusion: It is recommended to determine one’s own laboratory-specific, method-specific, trimester-wise RIs for maternal thyroid hormone status and use them for screening of pregnant women.

Keywords: Enzyme-linked immunosorbent assay, Laboratory research, Pregnancy, Reference interval, Thyroid hormones, Trimester specific.

How to cite this article: Chakrabarty BK, Mitra B, Pal R, Hazra N. Specific Reference Intervals of Serum Triiodothyronine, Thyroxine, and Thyroid-stimulating Hormone in Normal Pregnant Indian Women as per Trimester. Indian J Med Biochem 2017;21(1):17-21.

Source of support: Nil

Conflict of interest: None

 
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